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Mar 10, 2021

Ct, Cq, Rn value in qRT-PCR

What Is a Cq (Ct) Value?

 https://bitesizebio.com/24581/what-is-a-ct-value/

Ct – threshold cycle
Cp – crossing point
TOP – take-off point
Cq – quantification cycle 


Image of a qPCR graph showing how the Cq value is obtained

Good summary for qRT-PCR

https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-learning-center.html 

 

https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/PG1503-PJ9169-CO019879-Re-brand-Real-Time-PCR-Understanding-Ct-Value-Americas-FHR.pdf

 

Rn value

https://www.qiagen.com/us/resources/faq?id=ee18399a-b88b-43ef-9929-27d79ef9ed09&lang=en

 The Rn value, or normalized reporter value, is the fluorescent signal from SYBR Green normalized to (divided by) the signal of the passive reference dye for a given reaction. The delta Rn value is the Rn value of an experimental reaction minus the Rn value of the baseline signal generated by the instrument. This parameter reliably calculates the magnitude of the specific signal generated from a given set of PCR conditions. For more information, please refer to your cycler's user manual.

 

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