Positional sequence bias in random primed libraries (5' bias in RNA-seq)

Positional sequence bias in random primed libraries

https://sequencing.qcfail.com/articles/positional-sequence-bias-in-random-primed-libraries/

  

RNA-Seq library with random primer has biased seqeunce in 5' region.

Trimming 5' does not solve this issue

This bias does not seem to have any serious problems for downstream analysis

If some libraries do not show this bias among RNA-seq libraries, those libraries might need double check for quality control.