HiSeq, MiSeq, NovaSeq : Read length, # of cluster per lane, Base per lane

https://medicine.yale.edu/keck/ycga/sequencing/illumina/hiseq.aspx

Sequencer	Read length	# of Clusters per lane (millions)	Bases per lane (Gbp)
HiSeq 2500 Rapid	1x75	150	11.25
HiSeq 2500 Rapid	2x75	150	22.5
HiSeq 2500 Rapid	2x150	150	45
HiSeq 2500 High-output	1x75	200	15
HiSeq 2500 High-output	2x75	200	30
HiSeq 4000	2x100	300	60
HiSeq 4000	2x150	300	90
NovaSeq S2	2x100	1650	330
NovaSeq S2	2x150	1650	500
NovaSeq S4	2x150	2000	600

https://www.fasteris.com/dna/?q=node/41

Illumina HiSeq Services:

Service Type	Run (Mode)	Number of pass filter clusters (1)	Yield (1)	Sequencing Run Time (2)
HiSeq 4000 (3) - 1x lane	1x50 bp	Up to 350 million, average yield 280-300 mio (*)	17.5 Gb	1 - 4 days
	1x150 bp		52.5 Gb
	2x75 bp		52.5 Gb
	2x150 bp		105 Gb
HiSeq 2500, v4 (4)  - 1x lane	2x125 bp	Up to 280 million, average yield 240-260 mio (*)	70 Gb	8 days
HiSeq 2500 Rapid Run, v2 - 1x flow cell run	1x50 bp	Up to 300 million, average yield 240-260 mio (*)	15 Gb	1 - 3 days
	1x125 bp		37.5 Gb
	2x50 bp		30 Gb
	2x125 bp		75 Gb
	2x250 bp		150 Gb
	2x300 bp		180 Gb
1x test run (done on MiSeq Nano) (5)	1x50 bp	500'000 to 1 million reads	50 Mb	+ 4 days
1x test run (done on MiSeq Nano) (5)	1x150 bp	500'000 to 1 million reads	150 Mb	+ 4 days
(1) All the calculations have been made with specifications and typical Fasteris results when using optimized loading conditions. Individual results may vary. (2) This time is the "pure" running time of the system and includes time for washing. Not included is the time for doing the entry QC, library preparation, collection of other lane projects and/or the time for de-multiplexing of the derived data files. (3) On HiSeq 4000, we typically can reach 300-320 million passed-filter DNA clusters, up to 350 million or more pass filter DNA clusters per lane. (4) On HiSeq 2500 we typically can reach 250-280 million passed-filter DNA clusters per lane. For libraries with a test run done, we guarantee a minimum of 250 million pass filter DNA clusters per lane. (5) A test sequencing run is an extra service step needed to be ordered when min guaranteed data output and min guaranteed data yield is needed, especially for sequencing of customer-provided ready-to-run (RTR) libraries. (*) Average yields are given by statistic analysis of our runs; not included are biased or non-standard samples. Conditions and Details are subject to changes without notice.

https://www.illumina.com/systems/sequencing-platforms/miseq/specifications.html

Cluster Generation and Sequencing

	MiSeq Reagent Kit v2				MiSeq Reagent Kit v3
Read Length	1 × 36 bp	2 × 25 bp	2 × 150 bp	2 × 250 bp	2 × 75 bp	2 × 300 bp
Total Time*	~4 hrs	~5.5 hrs	~24 hrs	~39 hrs	~21 hrs	~56 hrs
Output	540–610 Mb	750–850 Mb	4.5–5.1 Gb	7.5–8.5 Gb	3.3–3.8 Gb	13.2–15 Gb

	MiSeq Reagent Kit v2 Micro	MiSeq Reagent Kit v2 Nano
Read Length	2 × 150 bp	2 × 250 bp	2 × 150 bp
Total Time*	~19 hrs	~28 hrs	~17 hrs
Output	1.2 Gb	500 Mb	300 Mb

* Total time includes cluster generation, sequencing, and base calling on a MiSeq System enabled with dual-surface scanning.

Reads Passing Filter^**

	MiSeq Reagent Kit v2	MiSeq Reagent Kit v3	MiSeq Reagent Kit v2 Micro	MiSeq Reagent Kit v2 Nano
Single Reads	12-15 million	22–25 million	4 million	1 million
Paired-End Reads	24–30 million	44–50 million	8 million	2 million

  ** Install specifications based on Illumina PhiX control library at supported cluster densities (865-965 k/mm² clusters passing filter for v2 chemistry and 1200-1400 k/mm² clusters passing filter for v3 chemistry). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter.