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Aug 4, 2015

things to install for NGS analysis

NCURSE -programming library for text-based user interfaces
 http://codybonney.com/installing-the-ncurses-library-in-centos-6-5/

 sudo yum install ncurses-devel


Cutadapt - adapter sequence trimming
 http://cutadapt.readthedocs.org/en/latest/index.html

Trimmomatic: A flexible read trimming tool for Illumina NGS data
 http://www.usadellab.org/cms/?page=trimmomatic

FastQC - quality check of fastq file
 http://www.bioinformatics.babraham.ac.uk/projects/fastqc/

Bowtie - short read aligner
 http://bowtie-bio.sourceforge.net/index.shtml
 http://bowtie-bio.sourceforge.net/bowtie2/index.shtml

Tophat - splice junction mapper for RNA-Seq reads (need Bowtie)
 https://ccb.jhu.edu/software/tophat/index.shtml

samtools - tools for manipulating alignments in the SAM(Sequence Alignment/Map) format,
 http://samtools.sourceforge.net/

picard -  tools for manipulating high-throughput sequencing (HTS) data and formats such as SAM/BAM/CRAM and VCF
 http://broadinstitute.github.io/picard/

HTSeq - Python package that provides infrastructure to process data from high-throughput sequencing assays
 http://www-huber.embl.de/users/anders/HTSeq/doc/overview.html

MACS - Model-based Analysis for ChIP-Seq
 http://liulab.dfci.harvard.edu/MACS/

PeakSplitter - Subdivision of ChIP-seq/ChIP-chip regions into discrete signal peaks
 http://www.ebi.ac.uk/research/bertone/software

R -  free software environment for statistical computing and graphics
 https://www.r-project.org/

IGV - Integrative Genomics Viewer
 https://www.broadinstitute.org/igv/
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